OrienTM [1, 2] is a computer software that utilizes an initial definition of transmembrane segments to predict the topology of transmembrane proteins from their sequence. It uses position-specific statistical information for amino acid residues which belong to putative non-transmembrane segments derived from statistical analysis of non-transmembrane regions of membrane proteins stored in the SwissProt database. Its accuracy compares well with that of other popular existing methods.


OrienTM is a Web-based tool that can be executed on the Biophysics and Bioinformatics Laboratory of the University of Athens's site.

1. Liakopoulos T, Pasquier C, Hamodrakas S: OrienTM: A novel method to predict transmembrane protein topology. In 21st conference of the hellenic society for biological sciences. Galissas, Syros island; 1999.

2. Liakopoulos T, Pasquier C, Hamodrakas S: A novel tool for the prediction of transmembrane protein topology based on a statistical analysis of the SwissProt database: the OrienTM algorithm. Protein Engineering Design and Selection 2001, 14:387–390.